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dc.coverage.spatialHEIDELBERG
dc.creatorLOPEZ-RIVERA, AMERICO ENRIQUE
dc.creatorSUAREZ-ISLA, BENJAMIN ALVARO
dc.creatorAMANDI, M.
dc.creatorBEAUDRY, C.
dc.creatorEILERS, P.
dc.creatorFUREY, A.
dc.creatorHALL, S.
dc.creatorJAMES, K.
dc.date.accessioned2017-04-27T18:49:15Z
dc.date.available2017-04-27T18:49:15Z
dc.date.issued2005
dc.identifier.issn1618-2642
dc.identifier.urihttp://hdl.handle.net/10533/196818
dc.description.abstractDomoic acid (DA) is a naturally-occurring amino acid that causes a form of human intoxication called amnesic shellfish poisoning (ASP) following the consumption of shellfish. A rapid and sensitive HPLC-UV method has been developed for analysis of DA and analogues in shellfish without the need for SPE clean-up. Isocratic chromatographic separation of DA and its isomers from shellfish matrix interferences and from the prevalent amino acid, tryptophan, was achieved by careful control of the mobile phase pH. The optimised pH was found to be 2.5 when using a Luna(2) C-18 column. Sample extraction was verified with control extracts from shellfish spiked at 5.0 and 10.0 mu g/g of DA and with certified reference material. The average extraction efficiency was 98.5%. The calibration, based on mussel tissue spiked with DA standard, was linear in the range 0.05-5.0 mu g/ml (r=0.9999) and the detection limit (signal:noise 3:1) was better than 25 ng/ml. The DA assay achieved good precision; %RSD=1.63 (intra-day, n=6) and %RSD=3.7 (inter-day, n=8). This method was successfully applied to a variety of shellfish species, allowing the rapid screening of a large number of samples per day (20-30), without the need for SPE clean-up. Quantitative data were obtained for shellfish samples containing domoic acid in the concentration range 0.25-330 mu g/g. Using the same chromatographic conditions, LC-MS3 was used to determine DA and its isomers, isodomoic acid D and epi-domoic acid, in scallop tissues.
dc.language.isoENG
dc.publisherSPRINGER HEIDELBERG
dc.relationinstname: Conicyt
dc.relationreponame: Repositorio Digital RI2.0
dc.relationinstname: Conicyt
dc.relationreponame: Repositorio Digital RI2.0
dc.relation.urihttps://doi.org/10.1007/s00216-005-3109-4
dc.titleImproved high-performance liquid chromatographic method for the determination of domoic acid and analogues in shellfish: effect of ph
dc.typeArticulo
dc.bibliographicCitation.stpage1540
dc.bibliographicCitation.endpage1545
dc.identifier.folioMR02I1004
dc.country.isoDEU
dc.description.citasisi18
dc.description.conicytprogramFONDEF
dc.description.emailamlopez@med.uchile.cl
dc.description.investmentarticle0
dc.description.number8
dc.description.volume381
dc.identifier.isiWOS:000228728200009
dc.relation.projectidinfo:eu-repo/grantAgreement/Fondef/MR02I1004
dc.relation.setinfo:eu-repo/semantics/dataset/hdl.handle.net/10533/93477
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.title.journalANALYTICAL AND BIOANALYTICAL CHEMISTRY (PRINT)
dc.title.journalabbreviationAnal. Bioanal. Chem.
dc.type.driverinfo:eu-repo/semantics/article
dc.description.shortconicytprogramFONDEF
dc.identifier.eissn0
dc.description.agrad0
dc.type.openaireinfo:eu-repo/semantics/publishedVersion


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